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KMID : 0378019730160040071
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New Medical Journal
1973 Volume.16 No. 4 p.71 ~ p.77
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Effect of Reserpine on Mast Cells in mesentery of Rats
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Abstract
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This experiment was performed in order to clarify morphologically the effect of reserpine, a prominent releaser of 5-hydroxytryptamine, on mast cells in mesentery of rats.
Healthy adult male rats weighing about 200g each used as experimental animals, and divided into normal and reserpine-treated groups. Reserpine was intraperitoneally injected in a single dose of 5mg/kg body weight to each animal and the reserpine-treated rats were sacrificed on the 3rd, 6th, and 12th hour and 1st, 2nd, 5th, 7th, 10th, 14th, 28th, and 40th day after the injection.
The mesenteries from the experimental animals were fixed in 10% neutral formalin solution in toto and stained with 0.1% toluidine blue solution. Numerical changes and morphological alteration, espe￢cially of the specific granules, of the cells in the experimental groups were observed.
Although insignificant fluctuations were noticed in some degree, number of the cells was not affected by reserpine during the experiment, but in the early stages after the reserpine-iniection, the mast cells showed irregularity in shape, slight reduction of the specific granules or increase of degranulated cells, and decrease of metachromatic reaction of the granules.
The above findings presumably indicate that reserpine do not play a specific role in degranulation or release of the granules of mast cells in the rat mesentery.
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